Wnt and Neuregulin1/ErbB signalling extends 3D culture of hormone responsive mammary organoids
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Wnt and Neuregulin1/ErbB signalling extends 3D culture of hormone responsive mammary organoids
The development of in vitro culture systems quantitatively and qualitatively recapitulating normal breast biology is key to the understanding of mammary gland biology. Current three-dimensional mammary culture systems have not demonstrated concurrent proliferation and functional differentiation ex vivo in any system for longer than 2 weeks. Here, we identify conditions including Neuregulin1 and...
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متن کاملIsolation, culture, and transfection of primary mammary epithelial organoids Primary mammary epithelial organoids were prepared from 8-week-old CD1 mice (Charles
Supplementary Experimental Procedures Isolation, culture, and transfection of primary mammary epithelial organoids Primary mammary epithelial organoids were prepared from 8-week-old CD1 mice (Charles River) as previously described (Nelson et al., 2006). Inguinal glands were removed aseptically, minced with razor blades, and incubated with agitation (350 rpm) at 37°C in DMEM/F12 supplemented wit...
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ژورنال
عنوان ژورنال: Nature Communications
سال: 2016
ISSN: 2041-1723
DOI: 10.1038/ncomms13207